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When the sample containing Leptin is added to the plate
Their extracellular domains are heavily glycosylated
The plate has been pre-coated with Mouse ENPP1 antibody
And then biotinylated Rat DEFB2 Antibody is added and binds to DEFB2 in the sample
And then biotinylated Human MMP-9 Antibody is added and binds to MMP-9 in the sample
Human CTSL2 (Cathepsin L2) ELISA Kit Packaging:96 wells When the sample containing LeptinThe Human CTSL2 (Cathepsin L2) ELISA Kit measures CTSL2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CTSL2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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