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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SP-D
The concentration of Human IL-2sRa/CD25 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human ACTN3
The plate has been pre-coated with Mouse EDN2 antibody
The absorbance is measured on a microplate reader at 450 nm
Mouse SYP (Synaptophysin) ELISA Kit SCYB4 Standards or samples are addedThe Mouse (SYP) Synaptophysin ELISA Kit measures Synaptophysin in Mouse samples. The plate has been pre coated with Mouse SYP antibody. SYP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SYP Antibody is added and binds to SYP in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SYP antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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