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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MRF
only those wells that contain Hamster IL10
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CHIT1
The OD value isproportional to the concentration of Human IL-6
Then a biotinylated detection antibody specific for Mouse RBP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human FLOT1 (Flotillin-1) ELISA Kit PADGEM Standards or samples are addedThe Human (FLOT1) Flotillin 1 ELISA Kit measures Flotillin 1 in samples. The plate has been pre coated with Human FLOT1 antibody. FLOT1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human FLOT1 Antibody is added and binds to FLOT1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FLOT1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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