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And then biotinylated Human PTPN22 Antibody is added and binds to PTPN22 in the sample
it binds with CDH3 bound to precoated antibody
And then biotinylated Mouse betaGD Antibody is added and binds to betaGD in the sample
Substrate solution is then added and color develops in proportion to the amount of Mouse FN1
Then Streptavidin-HRP is added and binds to the Biotinylated CLEC7A antibody
Rat CNP (C-Type Natriuretic Peptide) ELISA Kit Packaging:96 wells And then biotinylated Human PTPN22The Rat CNP (C Type Natriuretic Peptide) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat CNP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Rat CNP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added.