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Then Streptavidin-HRP is added and binds to the Biotinylated F10 antibody
The OD value is proportional to the concentration of Guinea pig IL-13
Then a biotinylated detection antibody specific for Mouse MIP-3Beta/ELC/CCL19 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
QRFP present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IL18
Human MLCK/MYLK (Myosin Light Chain Kinase) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human MLCK MYLK (Myosin Light Chain Kinase) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human MLCK MYLK. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MLCK MYLK and Avidin Horseradish Peroxidase (HRP) conjugate are added