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The concentration of EPI in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated ITIH3 antibody
The assay plate has been pre-coated with mouse anti-Human SCGN monoclonal antibody
The plate has been pre-coated with Rat CHRM3 antibody
And then biotinylated Mouse TLR5 Antibody is added and binds to TLR5 in the sample
Human MC5R (Melanocortin Receptor 5) ELISA Kit Packaging:96 wells The concentration of EPI inThe Human (MC5R) Melanocortin Receptor 5 ELISA Kit measures Melanocortin Receptor 5 in samples. The plate has been pre coated with Human MC5R antibody. MC5R present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MC5R Antibody is added and binds to MC5R in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MC5R antibody. After incubation unbound Streptavidin HRP is washed away