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The concentration of Human NGAL in the samples is then determined by comparing the OD of the samples to the standard curve
then added a HRP-conjugated antibody specific to Human BNP
You can calculate the concentration of Human LEP in the samples by comparing the OD of the samples to the standard curve
You can calculate the concentration of Human SP-D in the samples by comparing the OD of the samples to the standard curve
Only those wells that contain Mouse REN
Human VIP (Vasoactive Intestinal Peptide) ELISA Kit AP2 The concentration of Human NGALThe Human VIP (Vasoactive Intestinal Peptide) ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Human VIP. During the reaction, Human VIP in the sample or standard competes with a fixed amount of Human VIP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human VIP. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish
