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DCT present in the sample is added and binds to antibodies coated on the wells
And then biotinylated Rat A1M Antibody is added and binds to A1M in the sample
Substrate solution is then added and color develops in proportion to the amount of Rat EPHX1
And then biotinylated Human GATM Antibody is added and binds to GATM in the sample
The Human Oncostatin M ELISA kit is a sandwich enzyme immunoassay
Rat FBP1 (Fructose-1, 6-bisphosphatase 1) ELISA Kit Packaging:96 wells DCT present in the sampleThe Rat (FBP1) Fructose 1, 6 bisphosphatase 1 ELISA Kit measures Fructose 1,6 Bisphosphatase 1 in Rat samples. The plate has been pre coated with Rat FBP1 antibody. FBP1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat FBP1 Antibody is added and binds to FBP1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FBP1 antibody. After incubation unbound Streptavidin HRP is washed