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Then a biotinylated detection antibody specific for Rat TNF-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
When the sample containing CD97 is added to the plate
only those wells that contain Human TGFb1
only those wells that contain Zebrafish IL10
The concentration of Sheep IFNa in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse MIP1a (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit (Small Sample) Packaging:48 wells Then a biotinylated detection antibodyThe Mouse MIP1a (Macrophage Inflammatory Protein 1 Alpha) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse MIP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse MIP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After
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