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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PTX3
Then Streptavidin-HRP is added and binds to the Biotinylated AMACR antibody
Substrate solution is then added and color develops in proportion to the amount of Human ENO3
it binds with STX12 bound to precoated antibody
The concentration of Human CKM in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse PDI (Protein Disulfide Isomerase) ELISA Kit Packaging:96 wells Standards or samples are addedThe Mouse PDI (Protein Disulfide Isomerase) ELISA Kit measures PDI in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PDI antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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