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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UCP2
only those wells that contain Rat VEGF121
CUBN present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Human HYAL2
The plate has been pre-coated with Rat TNMD antibody
Human TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human TNF Alpha (Tumor Necrosis Factor Alpha) ELISA Kit measures TNF Alpha in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TNF antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
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