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mixing with PCR-amplified fragments that are completely homologous to the vector (15-40 bp) and reacting at 37C
The concentration of Guinea pig RANTES in the samples is then determined by comparing the OD of the samples to the standard curve
The OD value is proportional to the concentration of Human IL-8
The OD value is proportional to the concentration of Mouse IL-12
Only those wells that contain Mouse FG
Human FGF-18 ELISA Kit BLCPM mixing with PCR-amplified fragments thatThe Human FGF 18 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human FGF18. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human FGF18. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those
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