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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IREB2
The concentration of Human IL-6R in the samples is then determined by comparing the OD of the samples to the standard curve
a fixed amount of HRP-labeled E2 competes with the endogenous E2 in the standard
The OD value is proportional to the concentration of ApoA1
only those wells that contain Mouse TUBa4A
Human HTN1 (Histatin-1) ELISA Kit Packaging:96 wells Standards or samples are addedThe Human (HTN1) Histatin 1 ELISA Kit measures Histatin 1 in samples. The plate has been pre coated with Human HTN1 antibody. HTN1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HTN1 Antibody is added and binds to HTN1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HTN1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.