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Then a biotinylated detection antibody specific for Rat TNF-alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
coli expression system and the target gene encoding Cys24-Glu188(Lys46Arg) is expressed
coli expression system and the target gene encoding Ser2-Ser110 is expressed with a 6His tag at the C-terminus
extracellular matrix proteins with high tensile strength and are the major components of connective tissue
You can calculate the concentration of Human C3 in the samples by comparing the OD of the samples to the standard curve
Guinea Pig CCL5/RANTES ELISA Kit Packaging:48 wells Then a biotinylated detection antibodyThe Guinea Pig CCL5 RANTES ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Guinea pig RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Guinea pig RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
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