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Substrate solution is then added and color develops in proportion to the amount of Rat Oprm1
Then Streptavidin-HRP is added and binds to the Biotinylated ELAVL1 antibody
Only those wells that contain Human ACE
And then biotinylated Rat UCHL1 Antibody is added and binds to UCHL1 in the sample
If the sample contains AgRP
Guinea pig ATF7 (Activating Transcription Factor 7) ELISA Kit PUMP-1 Substrate solution is then addedThe Guinea pig ATF7 (Activating Transcription Factor 7) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Guinea pig ATF7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Guinea pig ATF7 and Avidin Horseradish Peroxidase (HRP) conjugate are added
