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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ARC
And then biotinylated Human RCAN1 Antibody is added and binds to RCAN1 in the sample
it binds with ROBO4 bound to precoated antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse MC4R
it binds with PON2 bound to precoated antibody
SP (Substance P) ELISA Kit Packaging:96 wells The microtiter plate provided inThe SP (Substance P) ELISA Kit measures SP in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with SP. SP in the sample or standard competes with a fixed amount of biotin labeled SP for sites on a pre coated antibody specific to SP. Free components are washed off. After adding HRP
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