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Substrate solution is then added and color develops in proportion to the amount of Mouse RARa
Then Streptavidin-HRP is added and binds to the Biotinylated CA12 antibody
Substrate solution is then added and color develops in proportion to the amount of Human SNX11
And then biotinylated Canine Renin Antibody is added and binds to Renin in the sample
SLC38A5 present in the sample is added and binds to antibodies coated on the wells
Human SEMA5A (Semaphorin-5A) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human SEMA5A (Semaphorin 5A) ELISA Kit measures SEMA5A in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SEMA5A antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
