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And then biotinylated Mouse VEGFD Antibody is added and binds to VEGFD in the sample
Then a biotinylated detection antibody specific for Porcine CNX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated ADNP antibody
And then biotinylated Human TGFB1 Antibody is added and binds to TGFB1 in the sample
The OD value is proportional to the concentration of Human PCⅠ
Rat MDH1 (Malate Dehydrogenase 1) ELISA Kit Packaging:48 wells And then biotinylated Mouse VEGFDThe Rat MDH1 (Malate Dehydrogenase 1) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat MDH 1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MDH 1 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
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