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a horseradish peroxidase conjugated mouse anti-Human Marapsin Antibody is added to the wells and binds to Marapsin in the sample
The OD value is proportional to the concentration of Chicken P-Cadherin
Substrate solution is then added and color develops in proportion to the amount of Human FABP9
You can calculate the concentration of Chicken CX40 in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Rabbit C2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat SEMA7A (Semaphorin 7A) ELISA Kit Packaging:48 wells a horseradish peroxidase conjugated mouseThe Rat SEMA7A (Semaphorin 7A) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat SEMA7A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SEMA7A and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well
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