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Substrate solution is then added and color develops in proportion to the amount of Human CLEC9A
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ARC
Substrate solution is then added and color develops in proportion to the amount of Human AMTN
Ddit3 present in the sample is added and binds to antibodies coated on the wells
The concentration of Rat NF-κBp105 in the samples is then determined by comparing the OD of the samples to the standard curve
Rat LCN2 (Lipocalin 2) ELISA Kit THBGB1 Substrate solution is then addedThe Rat (LCN2) Lipocalin 2 ELISA Kit measures Lipocalin 2 in Rat samples. The plate has been pre coated with Rat LCN2 antibody. LCN2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LCN2 Antibody is added and binds to LCN2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LCN2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
