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100 ul Testosterone-HRP conjugate reagent at room temperature for 60 minutes
The concentration of Mouse DEFa5 in the samples is then determined by comparing the OD of the samples to the standard curve
a horseradish peroxidase conjugated mouse anti-Mouse CD157 Antibody is added to the wells and binds to CD157 in the sample
The Mouse ECM1 (Extracellular Matrix Protein 1) ELISA Kit is a Sandwich enzyme immunoassay
only those wells that contain TGFb3
Human CD226 (Cd226 Antigen) ELISA Kit NIF 100 ul Testosterone-HRP conjugate reagentThe Human (CD226) Cd226 Antigen ELISA Kit measures Cd226 Antigen in samples. The plate has been pre coated with Human CD226 antibody. CD226 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CD226 Antibody is added and binds to CD226 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CD226 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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