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And then biotinylated Mouse ATG4D Antibody is added and binds to ATG4D in the sample
only those wells that contain Human TPPP
only those wells that contain Human ATG7
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 43 nm
The assay plate has been pre-coated with mouse anti-Mouse IL-6R monoclonal antibody
Human C5A (Complement Component 5a) ELISA Kit PBSF And then biotinylated Mouse ATG4DThe Human C5A (Complement Component 5a) ELISA Kit measures C5a in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti C5a antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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