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PLA2G10 present in the sample is added and binds to antibodies coated on the wells
The plate has been pre-coated with Mouse CHI3L1 antibody
Then a biotinylated detection antibody specific for Human MR-ProADM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of APOL1 in the sample by plotting standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated SPX antibody
Rat AGRP (Agouti Related Protein) QuickTest ELISA Kit Packaging:96 wells PLA2G10 present in the sampleThe Rat AGRP (Agouti Related Protein) QuickTest ELISA Kit measures AgRP in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into
