Rat CYP27A1 (Cytochrome P450 27A1) ELISA Kit (Small Sample) GRIPGHS Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TUBb1

Then a biotinylated detection antibody specific for Mouse MUC5B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LPO

A set of calibrators is used to plot a calibrator curve from which the amount of pregnenolone in specimen samples and controls can be directly read

only those wells that contain Human CHI3L1

Rat CYP27A1 (Cytochrome P450 27A1) ELISA Kit (Small Sample) GRIPGHS Standards or samples are addedThe Rat CYP27A1 (Cytochrome P450 27A1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat CYP27A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat CYP27A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate