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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MIF
it binds with GDF5 bound to precoated antibody
If the sample contains UGP2
Then Streptavidin-HRP is added and binds to the Biotinylated ENTPD4 antibody
The concentration of MMP-9 in the sample was calculated by drawing a standard curve
VB6 (Vitamin B6) ELISA Kit Packaging:48 wells Standards or samples are addedThe VB6 (Vitamin B6) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with VB6. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to VB6. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme substrate reaction
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