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Then a biotinylated detection antibody specific for Human HMGB-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of PRX in the sample by plotting standard curve
5-Bisphosphate Phosphodiesterase Beta-3 in samples
a capture antibody-SNAP25-biotin-detection antibody complex was formed
it binds with NBN bound to precoated antibody
Mouse TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit OCIF Then a biotinylated detection antibodyThe Mouse TFF3 (Trefoil Factor 3, Intestinal) ELISA Kit measures TFF3 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti TFF3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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