Human DPT (Dermato-Pontin) ELISA Kit BSF-1 Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for sST2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL32

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGa11

The OD value is proportional to the concentration of Human IL-1ra/IL-1F3

Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Mouse IgG1 are added to the micro ELISA plate wells

Human DPT (Dermato-Pontin) ELISA Kit BSF-1 Then a biotinylated detection antibodyThe Human (DPT) Dermato Pontin ELISA Kit measures Dermato Pontin in samples. The plate has been pre coated with Human DPT antibody. DPT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human DPT Antibody is added and binds to DPT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated DPT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.