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The concentration of Rat VASP in the samples is then determined by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human FOXO1
The concentration of Human SLC16A1 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Mouse NRP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ESM1
Mouse KLK1 (Kallikrein-1) ELISA Kit Packaging:96 wells The concentration of Rat VASPThe Mouse (KLK1) Kallikrein 1 ELISA Kit measures Kallikrein 1 in Mouse samples. The plate has been pre coated with Mouse KLK1 antibody. KLK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse KLK1 Antibody is added and binds to KLK1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated KLK1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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