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Then Streptavidin-HRP is added and binds to the Biotinylated DEFB3 antibody
Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DKK3
Then a biotinylated detection antibody specific for Human CP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Human bFGF/FGF2
Rat NPHN (Nephrin) ELISA Kit SERPING1 Then Streptavidin-HRP is added andThe Rat (NPHN) Nephrin ELISA Kit measures Nephrin in Rat samples. The plate has been pre coated with Rat NPHS1 antibody. NPHS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NPHS1 Antibody is added and binds to NPHS1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NPHS1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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