Human SEMA4D (Semaphorin 4D) ELISA Kit HO1 the TMB substrate (enzyme substrate)

the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of 3alpha-Diol G present

The OD value is proportional to the concentration of Human IFN Beta

Then Streptavidin-HRP is added and binds to the Biotinylated CA9 antibody

And then biotinylated Human AGMO Antibody is added and binds to AGMO in the sample

And then biotinylated Human PCYOX1 Antibody is added and binds to PCYOX1 in the sample

Human SEMA4D (Semaphorin 4D) ELISA Kit HO1 the TMB substrate (enzyme substrate)The Human (SEMA4D) Semaphorin 4D ELISA Kit measures Semaphorin 4D in samples. The plate has been pre coated with Human SEMA4D antibody. SEMA4D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SEMA4D Antibody is added and binds to SEMA4D in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA4D antibody. After incubation unbound Streptavidin HRP is washed away during a