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Substrate solution is then added and color develops in proportion to the amount of Mouse CCL2
And then biotinylated Human AMTN Antibody is added and binds to AMTN in the sample
Then a biotinylated detection antibody specific for Mouse MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The OD value is proportional to the concentration of IFN-Gamma
The concentration of GMPS in the sample was calculated by drawing a standard curve
Human H3cit-NE (neutrophil extracellular trap) ELISA Kit HCHOLA3 Substrate solution is then added
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