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Then a biotinylated detection antibody specific for Human AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
You can calculate the concentration of Human I-TAC/CXCL11 in the samples by comparing the OD of the samples to the standard curve
The concentration of Human HVEM in the samples is then determined by comparing the OD of the samples to the standard curve
The Human (CX3CR1) Cx3C-Chemokine Receptor 1 ELISA Kit measures Cx3C-Chemokine Receptor 1 in samples
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 42 nm
Sheep EPO (Erythropoietin) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Sheep (EPO) Erythropoietin ELISA Kit measures Erythropoietin in Sheep samples. The plate has been pre coated with Sheep EPO antibody. EPO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Sheep EPO Antibody is added and binds to EPO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated EPO antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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