Rat TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit Packaging:48 wells Then Streptavidin-HRP is added and

Then Streptavidin-HRP is added and binds to the Biotinylated PRB1 antibody

it binds with JPT1 bound to precoated antibody

Then Streptavidin-HRP is added and binds to the Biotinylated RAD50 antibody

Then a biotinylated detection antibody specific for Human PROC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then Streptavidin-HRP is added and binds to the Biotinylated OSBP2 antibody

Rat TNF-Alpha (Tumor Necrosis Factor Alpha) ELISA Kit Packaging:48 wells Then Streptavidin-HRP is added andThe Rat TNF Alpha (Tumor Necrosis Factor Alpha) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat TNF Alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TNF Alpha and Avidin Horseradish Peroxidase (HRP) conjugate are added successively