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Then a biotinylated detection antibody specific for Mouse PGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of INT6 in the sample by plotting standard curve
And then biotinylated Human MXD1 Antibody is added and binds to MXD1 in the sample
it binds with IFNT1 bound to precoated antibody
only those wells that contain Human hs Ab1-40
Mouse IBSP (Bone sialoprotein 2) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse IBSP (Bone sialoprotein 2) ELISA Kit measures Ibsp in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Ibsp antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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