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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTSG
Then a biotinylated detection antibody specific for Mouse BMG/β2-MG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then a biotinylated detection antibody specific for Human SCCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human MSTN in the samples is then determined by comparing the OD of the samples to the standard curve
The Mouse TGFbR2 (Transforming Growth Factor Beta Receptor II) ELISA Kit is a Sandwich enzyme immunoassay
Bovine IFI30 (Gamma-Interferon-inducible Lysosomal Thiol Reductase) ELISA Kit ME Standards or samples are addedThe Bovine (IFI30) Gamma Interferon inducible Lysosomal Thiol Reductase ELISA Kit measures Gamma Interferon Inducible Lysosomal Thiol Reductase in Bovine samples. The plate has been pre coated with Bovine IFI30 antibody. IFI30 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine IFI30 Antibody is added and binds to IFI30 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated
