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Then a biotinylated detection antibody specific for Mouse ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 16 nm
You can calculate the concentration of Mouse ST2 in the samples by comparing the OD of the samples to the standard curve
And then biotinylated Human CHRM5 Antibody is added and binds to CHRM5 in the sample
A standard curve is prepared relating color intensity to the concentration of the Progesterone
Human CTRP3 (Complement C1q tumor necrosis factor-related protein 3) ELISA Kit Packaging:48 wells Then a biotinylated detection antibodyThe Human (CTRP3) Complement C1q tumor necrosis factor related protein 3 ELISA Kit measures C1q; TNF related protein 3 in samples.