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The Human GNLY (Granulysin) ELISA kit uses the Sandwich-ELISA principle
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 16 nm
Universal VD3 in the sample or standard competes with a fixed amount of Universal VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD3
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PDGFBB
The OD value is proportional to the concentration of Rat IgG2a
Human CBX1 (Chromobox Protein Homolog 1) ELISA Kit Cadherin 2 Type 1 The Human GNLY (Granulysin) ELISAThe Human (CBX1) Chromobox Protein Homolog 1 ELISA Kit measures Chromobox Protein Homolog 1 in samples. The plate has been pre coated with Human CBX1 antibody. CBX1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CBX1 Antibody is added and binds to CBX1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CBX1 antibody. After incubation unbound Streptavidin HRP is washed
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