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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIGLEC2
Then Streptavidin-HRP is added and binds to the Biotinylated MT-RNR1 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated MC3R antibody
And then biotinylated Human GPNMB Antibody is added and binds to GPNMB in the sample
If the sample contains HHIP
Rat ADCYAP1 (Pituitary adenylate cyclase-activating polypeptide) ELISA Kit Packaging:96 wells Standards or samples are addedThe Rat ADCYAP1 (Pituitary adenylate cyclase activating polypeptide) ELISA Kit measures ADCYAP1 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti Adcyap1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
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