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The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm
Only those wells that contain Human NGF
You can calculate the concentration of Mouse HMGB-1 in the samples by comparing the OD of the samples to the standard curve
only those wells that contain Human GH and HRP-conjugated antibody will exhibit a change in color
The concentration of Rat VEGF-A in the samples is then determined by comparing the OD of the samples to the standard curve
Rat IL-1Beta (Interleukin 1 Beta) ELISA Kit EasyStep The enzyme-substrate reaction is terminatedThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat IL 1Beta. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat IL 1Beta. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
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