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The concentration of Human AMIGO1 in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human MCP2 in the samples is then determined by comparing the OD of the samples to the standard curve
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 32 nm
GHRL present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Human ADAMTS5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human IKZF1 (DNA-binding Protein Ikaros) ELISA Kit THBS2 The concentration of Human AMIGO1The Human (IKZF1) DNA binding Protein Ikaros ELISA Kit measures DNA Binding Protein Ikaros in samples. The plate has been pre coated with Human IKZF1 antibody. IKZF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IKZF1 Antibody is added and binds to IKZF1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IKZF1 antibody. After incubation unbound Streptavidin HRP is
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