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Then a biotinylated detection antibody specific for Rat IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
the protein of interest is often expressed as a MBP-fusion protein
The OD value is proportional to the concentration of Human TRAIL/TNFSF10
The Human MIF (Macrophage Migration Inhibitory Factor) ELISA kit uses the Sandwich-ELISA principle
Then a biotinylated detection antibody specific for CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Rat CHGB (Chromogranin B) ELISA Kit (Small Sample) IL-1RT-2 Then a biotinylated detection antibodyThe Rat CHGB (Chromogranin B) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat CHGB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat CHGB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
