Human RELN (Reelin) ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human HPX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Only those wells that contain Rat BDNF

Then Streptavidin-HRP is added and binds to the Biotinylated ATF3 antibody

In the second incubation step

only those wells that contain Mouse ATG12

Human RELN (Reelin) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (RELN) Reelin ELISA Kit measures Reelin in samples. The plate has been pre coated with Human RELN antibody. RELN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RELN Antibody is added and binds to RELN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RELN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate