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Then a biotinylated detection antibody specific for Rat ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
and the color intensity is directly proportional to the amount of Human LAMP3 present
Then Streptavidin-HRP is added and binds to the Biotinylated EZH2 antibody
The plate has been pre-coated with Human CDH13 antibody
You can calculate the concentration of Human IL-2 in the samples by comparing the OD of the samples to the standard curve
Human MUC3B (Mucin-3B) ELISA Kit IRH Then a biotinylated detection antibodyThe Human MUC3B (Mucin 3B) ELISA Kit measures MUC3B in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti MUC3B antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.