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6His tag at the N-terminus
only those wells that contain GCSF
Calculate the concentration of ASBT in the sample by plotting standard curve
And then biotinylated Human HEYL Antibody is added and binds to HEYL in the sample
a horseradish peroxidase conjugated mouse anti-Human Fas Antibody is added to the wells and binds to Fas in the sample
Human CD177 (CD177 antigen) ELISA Kit HAG-2 6His tag at the N-terminusThe Human CD177 (CD177 antigen) ELISA Kit measures CD177 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CD177 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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