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Then a biotinylated detection antibody specific for Mouse G-CSF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
After 30 minutes incubation and the last washing step
The OD value is proportional to the concentration of Human MCSF
Real Time RT-PCR
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep IL10
LAMC1 (Laminin Gamma 1) ELISA Kit (Small Sample) Cadherin 5 Type 2 Then a biotinylated detection antibodyThe LAMC1 (Laminin Gamma 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human LAMC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human LAMC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,
