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F9 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Human EAF2
The concentration of Mouse FKBP1B in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ITGa1
Substrate solution is then added and color develops in proportion to the amount of Mouse DPP4
Rat NT-3 (Neurotrophin 3) ELISA Kit AQP4 F9 present in the sampleThe Rat (NT 3) Neurotrophin 3 ELISA Kit measures Neurotrophin 3 in Rat samples. The plate has been pre coated with Rat NT 3 antibody. NT 3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT 3 Antibody is added and binds to NT 3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NT 3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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