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The Human THP (Tamm-Horsfall Glycoprotein) ELISA kit uses the Sandwich-ELISA principle
Only those wells that contain Human IL-6
only those wells that contain Human IFN Gamma
You can calculate the concentration of Mouse KIM-1 in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human TARC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Avi-Tag Mouse mAb Packaging:50 uL The Human THP (Tamm-Horsfall Glycoprotein)The Avi tag is a biotin acceptor peptide1, GLNDIFEAQKIEWHE. The 15 residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.
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