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Substrate solution is then added and color develops in proportion to the amount of Human FGFBP1
The OD value is proportional to the concentration of Mouse PKIa
You can calculate the concentration of Human PIAS3 in the samples by comparing the OD of the samples to the standard curve
The concentration of Sectm1a in the sample was calculated by drawing a standard curve
Then a biotinylated detection antibody specific for Human ADP/Acrp30 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse PKCg (Protein Kinase C Gamma) ELISA Kit LSEL Substrate solution is then addedThe Mouse PKCg (Protein Kinase C Gamma) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse PKCg. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PKCg and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro
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