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Then a biotinylated detection antibody specific for Human MPIF2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Human BHLHE41 Antibody is added and binds to BHLHE41 in the sample
a horseradish peroxidase conjugated mouse anti-Human CD50 Antibody is added to the wells and binds to CD50 in the sample
Substrate solution is then added and color develops in proportion to the amount of Rat HTR2B
it binds with EIF5A bound to precoated antibody
Mouse CNN1 (Calponin-1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Mouse CNN1 (Calponin 1) ELISA Kit measures Cnn1 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CNN1 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
