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EPHB6 present in the sample is added and binds to antibodies coated on the wells
a horseradish peroxidase conjugated mouse anti-Human M-CSF Antibody is added to the wells and binds to M-CSF in the sample
Ntn1 present in the sample is added and binds to antibodies coated on the wells
it binds with IL-18 bound to precoated antibody
Then a biotinylated detection antibody specific for Human F7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human CPA3 (Mast cell carboxypeptidase A) ELISA Kit ZFGF5 EPHB6 present in the sampleThe Human CPA3 (Mast cell carboxypeptidase A) ELISA Kit measures CPA3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CPA3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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